Fabrication of Room-Temperature Phosphorescent Nanoparticles Assembled with Peptide-Aptamer
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Optical biosensors have been studied recently to detect bio-materials and lead to significant progresses in diagnosis and sensing. However, cellular damage and undesirable autofluorescence can be caused due to excitation. Many strategies have focused on choosing appropriate fluorophore whose emission wavelength is far from autofluorescence wavelength to avoid it. In this study, we used the phosphorescent material, platinum octaethylporphyrin (PtOEP), to recognize protein without autofluorescence. Herein, we fabricated PtOEP nanoparticles with hundreds of nanometers or tens of micrometers size by reprecipitation method or evaporation method. Several measurements such as optical microscopy, scanning electron microscope (SEM), UV/Vis absorption, photoluminescence, confocal laser scanning microscope (CLSM) of fabricated PtOEP nanoparticles were conducted. Self-assembled PtOEP nanoparticles with peptide aptamer A8/A17 showed photoluminescence intensity change after treatment with target protein heat shock protein70 (HSP70). It could be further applied to recognize specific proteins.