Improved Transfection of Human Mesenchymal Stem Cells using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System
발표자
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초록
내용
Human mesenchymal stem cells (hMSCs) have been studied in regenerative medicine and tissue engineering. However, hMSCs have some challenges because there is no suitable way to control their behavior in vivo, such as differentiation to a desired cell type. So we use a genetic modification and proper carrier to deliver the nucleic acid to control the cells. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Using the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic di erentiation of hMSCs.