Enhancement of stability of ceramide-based nanovesicles assembled by 1-O-acylceramide
발표자
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초록
내용
Stratum corneum (SC) lipids have intensively been applied as a component of nanovesicles for enhancing skin barrier function, but the structure of nanovesicles composed of SC lipids is unstable due to high packing parameter of ceramide. Herein, we propose an extremely stable SC mimetic-nanovesicle system in which 1-O-acylceramide (1OA) facilitates tight interaction between the multi-layers of SC lipid vesicle containing ceramide NP, stearic acid, and cholesterol, thus showing an improved long-term stability. Regardless of presence of 1OA in the nanovesicles, they maintained their multi-lamellar structure. However, the addition of 1OA in the nanovesicles affects the inter-spacing distance and ceramide crystallinity, attributed to the acyl chain conformation of 1OA. Also, we confirmed the ability of 1OA to strengthen skin barrier by analyzing western blot. These findings indicate that 1OA is important for stabilizing the structure of nanovesicles containing SC lipids.