ATP-Independent Intracellular Gene Delivery Mediated by polymeric biomimetics of amphiphilic Bile Acid-PEI
발표자
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초록
내용
Nanoscale molecular amphiphilic polymer conjugates, bile acid-polyethylenimine (BA-PEI), present efficient gene transfer route into mammalian cells across the cell membrane. This process doesn’t need the aid of endocytosis or other ATP-dependent processes, thus mimicking cell-penetrating peptides. The exposure of a hydrophilic face of the amphiphilic BA moiety on the surface of BA-PEI/DNA complex that mediates direct contact of the BA molecules to the cell surface seems to play an important role in the endocytosis- and energy-independent internalization process. The new modality of the polymeric biomimetics can be applied to enhanced delivery of macromolecular therapeutics.