Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Detection of Trace Proteins
발표자
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초록
내용
In this study, we developed protein-imprinted sensors, which were electrochemically fabricated on screen-printed carbon electrodes (SPCEs) for the cytokine detection. A double layer comprising poly(o-phenylenediamine) [P(o-PD)] and poly(chromotrope 2R) [P(C2R)] with a protein template (i.e., cytokine) as molecularly imprinted polymer (MIP) film was formed on the electrodes through cyclic voltammetry (CV). The electrochemical sensing properties were investigated via CV and electrochemical impedance spectroscopy to investigate the imprinting effect on the MIP films. The results showed that the MIP-based electrochemical sensor had a highly sensitive and selective response in the trace protein solution (a few pg/mL). Thus, these pretein sensing platforms based on the electropolymerized MIP films could be used in the practical detection of macromolecular proteins.