Electrophoretic determination of aggregation number of Pluronic F127
발표자
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초록
내용
Pluronic F127, poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), amphiphilic triblock copolymer can form nanosized micelle in aqueous environment above its critical micelle temperature (CMT) and critical micelle concentration (CMC). Under the condition a defined number of polymer is required to form a spherical micelle, which represents aggregation number (Naggre). In this study, we adopted electrophoresis to determine the Naggre of F127. Micelles consisting of pristine F127 and phosphorylated F127 (PP-F127) were blended in different volume ratio to control the number of surface charges per micelle. Furthermore, the core of micelle was stabilized to prevent exchange of individual polymer among micelles. To assess the role of surface charge coverage on electrophoretic mobility, agarose gel electrophoresis of the blends was performed. By analyzing discrete gel bands, Naggre of F127 we determined was 30.