“Turn-On” Detection of Human Epithelial Cell Using Aptamer/Graphene Oxide
발표자
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초록
내용
Aptamers are biopolymers, self-assemble ssDNA or RNA, which can bind to specific target. As well as antigen-antibody binding, aptamer has high affinity and specificity but also stability. Therefore, aptamer has a great potential to be used in many field, biosensor/chip, medical and science, etc. In this research, we developed one-step “turn-on” detectable biosensor based on aptamer/graphene oxide that can easily detect human epithelial cell. We selected Epithelial Growth Factor Receptor(EGFR) aptamer that could bind to the EGFR protein in the cell membrane. Graphene oxide acts as a sensing platform with high fluorescence quenching efficiency and realize real-time target monitoring in living cells. By utilizing the advantages of the above material, we amplified the efficiency to detect only the target material.