Charge-dependent cellular uptake of spherical polymeric nanoparticles
발표자
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초록
내용
Passive targeting is a strategy toward personalized medicine and cancer nanotherapy, where nano-sized delivery vehicles do not have any ligand for receptor-mediated endocytosis. In passive targeting, several parameters such as size, shape and charge govern the effectiveness of cellular uptake. Here, we present a method to control the density of charges on surface of nanoparticles(NPs). We prepared fluorescent NPs(d ＜ 20 nm) covered with 0 - 100% positive and negative charges in every 10% interval and characterized the NPs by dynamic light scattering, zeta potential and gel electrophoresis. Then passive cellular uptake in fourteen cell lines using the NPs was investigated by fluorescence microscopy. We found out that, three cell lines exhibit specific charge-dependent uptake, which also agree to flow cytometry. These findings may contribute to understand fundamental role of charges on NPs for applications in therapeutics and diagnostics.