Fabrication of Functionalized Porous Scaffold for Tissue Engineering
발표자
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초록
내용
Functional restoration of injured tissue has been studied variously in modern medicine. In this study, we fabricated porous scaffold that generate reactive oxygen species (ROS). Depending on the level of ROS, it affects the fate of the cell. High levels of ROS cause cellular oxidative stress leading to apoptosis. However, low levels of ROS provide beneficial effects on cell proliferation. Protoporphyrin IX (PpIX) was used as a photosensitizer for the generation of ROS after laser irradiation and grafted onto the scaffold surface. Schwann cells were incubated with various concentrations of PpIX and laser intensity to measure the optimal concentration of the ROS. As a result, we found optimal conditions for producing low levels of ROS, which had a positive effect on cell proliferation. Our results show that it is possible to produce advanced porous scaffold with great potential for tissue engineering.