Therapeutic protein & drug co-loaded silica nanoparticles for synergistic anticancer therapy
발표자
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초록
내용
Intracellular protein delivery has an important role, affecting reprogramming of the cell behavior. Despite of advantages of therapeutic proteins such as high activity and specificity, it is hard to deliver proteins due to low permeability, short in vivo half-life, and poor stability. Herein, we designed protein and anticancer drug co-loaded silica nanoparticle via in-situ reaction. β–galactosidase and methylene blue are chosen as model cargo molecules in a preliminary experiment. β–galactosidase and methylene blue-containing silica nanoparticle has structure including disulfide bonds. Biodegradability of silica nanoparticle can be controlled by the amount of disulfide containing molecules as disulfide bond is cleaved under GSH-rich tumor microenvironment. By introduction of targeting ligand using Michael-addition chemistry, protein and drug delivery nanosystem may have enhanced selectivity to tumor, leading to synergistic effect on killing cancer cells from protein and drugs.