Hydrogels have offered biological applications such as scaffold, implantable devices and bio-sensors due to their advantages including (i) easy bio-functional modification, (ii) high biocompatibility, and (iii) prevention of non-specific adsorptions. Inflammatory enzymes, such as MMP-9, have been investigated widely because of their roles in development of inflammation. MMP-9 cleaves specific peptide sequences during degradation of extracellular matrix. Herein, we developed MMP-9 activatable fluorogenic hydrogel intraocular lens sensor using MMP-9 targetable peptide probe. In detail, C-terminus of peptide probe was conjugated inside the PEGDAAm-co-PAlAm hydrogel by EDC/NHS chemistry. PEGDAAm hydrogel showed improved stability compared to PEGDA hydrogels. Peptide probe inside the hydrogel sensor was cleaved with existence of MMP-9, becoming fluorescence off-state into on-state by detachment of dye and quencher. Fluorescence intensity was increased with concentration of MMP-9 increased.