Release-controlable antibiotics via DNA minor groove binding and ionic coating methods
발표자
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초록
내용
DNA (Deoxyribonucleic acid) was founded as key-role factor that transfer genetic information in central dogma of molecular biology. However, previously in many research groups, DNA has been also re-discovered and engineered as bio-polymer to be used for biological and clinical applications, just like other biocompatable polymers. As polymer of nucleotide phosphate, DNA has binding sites that can attach specific small molecules stably (e.g. Intercalation, Major groove binding, Minor groove binding). In here, we estimated loading & releasing properties of well-known minor groove binding antibiotic, Netropsin. For the stable loading and releasing system, DNA microparticle (DNAmp) synthesized from rolling circle amplification (RCA) technique was used as the template. Moreover, we demonstrated that netropsin-loaded DNAmp (DNAmp@Ne) can be shielded by calcium ion coating to DNAmp, for long-term releasing system.