Fluorinated helical polypeptide for enhanced gene delivery
발표자
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초록
내용
Gene therapy has gained great attention for treatment of various diseases, including viral diseases, cancer, and genetic disorders. However, to achieve successful gene transfection, naked DNA has to pass several processes. Therefore, vector systems are required to attain high gene transfection efficiency. Even though viral vector showed high gene transfection efficiency, due to its inherent immunogenicity, numerous studies are focused on developing non-viral vectors. Among various candidates of non-viral vector, we utilized cationic helical polypeptide as a template. As one of a modification strategy of non-viral vector, fluorination is simple and potent method for gene transfection. Because of peculiar characteristic of fluorine, fluorination induced endosomal escape and efficient gene transfection. In this study, we introduced perfluoroalkyl chain into the cationic helical polypeptide to accomplish enhanced helicity and high gene transfection efficiency simultaneously.