The structural stability and biocompatibility of 3D gelatin scaffolds by cross-linking method
발표자
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초록
내용
Bioinks used in 3D bioprinting should have good biocompatibility and printability. Biocompatible and biodegradable gelatin is a good candidate bioink, but printability is poor due to low viscous of gelatin solution. In this study, we improved the printability of the gelatin by controlling the viscous of the gelatin solution. The 3D printed scaffolds were cross-linked using two types of different cross-linking methods (physical and chemical method). The structural stability of cross-linked 3D scaffolds was confirmed by SEM. The sizes of the chemical cross-linked 3D scaffolds were significantly reduced, but the physical cross-linked 3D scaffolds maintained their sizes (less than a 3% reduction). To evaluate the cell proliferation of the 3D scaffold, human dermal fibroblasts cultured on the 3D scaffold. After 14 days of culture, DNA assay showed that the cell proliferation rate of the physical cross-linked 3D scaffold was 44% higher than that of the chemical cross-linked 3D scaffold.