Photostable polymeric nanoparticle containing hydrophobic Lumogen<SUP>®</SUP> dye as cellular biomarkers
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초록
내용
Lumogen® fluorescent dyes have great light stability as well as high fluorescence quantum yields. Despite the excellent photophysical properties of dyes, the highly hydrophobic structure in common makes the compounds difficult to function as biological fluorescence probes. To generally utilize a family of organic fluorescent dyes such as Lumogen® Red, Orange, Yellow, and Violet, as a biomarker for cellular imaging in flow cytometry and fluorescence microscopy, we used an encapsulation method using amphiphilic block copolymer namely Pluronic® F127. The photostability of Lumogen nanoparticles were compared with commercial dye, Alexa488 and DAPI, used primarily bioimaging. Cell uptake of the probe nanoparticles by HeLa cells was analyzed by flow cytometry and confocal laser scanning microscopy. Also, we observed to confirm the position of the Lumogen nanoparticles in the HeLa cells using a high voltage electron microscope.