Calcium Peroxide-mediated Surface PEGylation for Preparing Low-binding Cell Culture Plates
발표자
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초록
내용
Various surface modification techniques have been developed to regulate surface properties of biomaterials. Recently, mussel-inspired adhesive molecules have attracted much attention because of their strong adhesion on various types of substrates. However, it is still challenging to enhance coating efficacy of the adhesive molecules owing to its insufficient oxidation by lack of oxygen (O2) during coating processes. Herein, we present a facile surface modification method using calcium peroxide (CP) that promote the coating efficiency of dopamine (DH) by O2 supply. We demonstrate that CP-mediated oxygen supply facilitated oxidation kinetics of DH, which improve coating efficacy of mussel-inspired biomolecules. Utilizing this system, we fabricate PEGylated surfaces to create low-binding cell culture plates, resulting in low cell attachment (5.26~9.65 %). In sum, our approach holds great potential as an advanced surface modification technique for a wide range of possible applications.