Administration of functional DNA nanoparticle for cancer immunotherapy
발표자
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초록
내용
Nucleic acids are one of basic components for living organisms. At the same time, DNAs and RNAs can be used as building blocks to build nanostructures using the sequence specific selfrecognition. The incorporation of lipid-modified nucleotides into DNA strands enables the formation of nano-size micelles. In a single self-assembly step, the micelles can be equipped with an immune adjuvant (CpG motifs) and an antigen peptide (OVA epitope) bearing peptide nucleic acid (PNA). In past research, we observed dose-dependent (CpG) activation of TLR-9 resulting in DC maturation in vivo. Here, we report the effect of the immunotherapeutic nucleic acid (INA) including immune-adjuvant and antigen peptide on the surface of lipid-modified DNA micelles in inhibition of melanoma tumor growth and its metastasis in the mouse model.