Cartilage regeneration using gellan gum/demineralized bone particles: In vitro and in vivo study
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초록
내용
Herein, DBPs incorporated GG (G/D) was fabricated to increase bioactivity in vitro, and enhance articular cartilage regeneration in vivo. The physical properties of the hydrogels were characterized by measuring ATR-FTIR, Bio-LV SEM, porosity and swelling ratio. Cell proliferation and chondrogenesis was determined by MTT assay, sulfated glycosaminoglycan (sGAG) content, and mRNA expression analysis. Furthermore, in vivo study was proceeded to confirm biocompatibility and articular cartilage regeneration ability of the DBPs/GG. Overall, the physiological examination presented that the hydrogel was compatible material to apply in cartilage TE and the in vitro analysis of an appropriate amount of DBPs exhibited highest cell growth, protein and mRNA expression. Moreover, in vivo study displayed biocompatibility and higher cartilage regeneration. ACKNOWLEDGMENT This research was supported by a grant of the Korea Health Technology R&D Project through KHIDI (HI15C2996)