Specific peptide ligand for targeting the light chain of BoNT/A
발표자
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초록
내용
The light chain (LC) of Botulinum neurotoxins (BoNTs) inhibits neurotransmitter release by destroying exocytotic fusion through cleavage of the soluble NSF (N-ethylmaleimide-sensitive fusion protein) attachment protein receptor (SNARE) proteins inside the cytosol and this is a key step to the development of botulism. The technical aim addressed in this study is to identify, characterize and evaluate peptide inhibitors for LC of BoNT serotype A to be utilized for future detection technology development and therapeutic treatments. This study demonstrates that the peptides developed from phage display technique are promising lead inhibitors, presenting the foundation for the generation of a more dominant derivative that can translate into rapid detection technology and therapeutic treatments. These findings also provide the proof of concept that peptide inhibitors targeting for LC and effectively blocking the endopeptidase activity of the toxin can be used as applicable BoNTs.