Synthesis and characterization of biocompatible glycol chitosan based nanoparticles for gene delivery
발표자
()
초록
내용
Since glycol chitosan is biocompatible and biodegradable, it has been studied as a good material as a gene delivery carrier. In this study, we have developed and studied a gene delivery carrier by introducing methyl acrylate and polyethylenimine using glycol chitosan. GMP800, GMP1300 and GMP2000 were identified by 1H-NMR and electrophoresis was performed to confirm the binding ability with pDNA. The particle size and surface charge of GMP800, GMP1300 and GMP2000 with pDNA were measured using a zetasizer. Cytotoxicity was assessed using various cell lines, and cellular uptake was confirmed by confocal microscopy. In addition, the gene expression rate in HeLa cell line was confirmed by confocal image through GFP, and transfection efficiency was measured by protein quantification. As a result, the prepared nanoparticles are expected to be used as biocompatible gene delivery carriers because of their low toxicity and strong binding force with genes.