PAMAM generation 3 by surface modification with KKHIK peptides for efficient gene delivery.
발표자
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초록
내용
Polyamidoamine (PAMAM) is one of the cationic polymers which is widely used for gene delivery because of its high density of cationic functional groups that can ionically bond with anionic plasmid DNA. However, due to the low gene delivery efficiency, it is difficult to apply and commercialize clinical applications. To overcome this problem, we introduced KKHIK sequence to the surface of PAMAM G3, which has been reported to increase the efficiency of introduction into the nucleus of cells. PAMAM G3-KKHIK showed improved transfection efficiency in HeLa and SW480 cell lines compared with that of unmodified PAMAM G3, and showed relatively low cytotoxicity. Therefore, introduction of KKHIK sequence to PAMAM shows potential by improving transfection efficiency as a novel gene carrier.