Synthesis and Characterization of Polyethylenimine Cross-linked by Oxidized Lactose for Gene Delivery Systems
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초록
내용
In this study, we synthesized polyethylenimine (PEI2k) cross-linked by oxidized lactose by reductive amination for efficient gene delivery systems. These polymers (LP2ks) synthesized with 3 kinds of mixing ratios, could condense pDNA into nano-sized polyplex particles with positive charges. LP2ks showed lower cytotoxicity than PEI25k in both Hela and A549 cell line. In serum condition, LP2ks displayed less decreased transfection efficiency than PEI25k, meaning a good serum-compatibility. It was found that LP2k polyplex could be internalized into cells efficiently by Flow cytometry results. Also, LP2k polyplex showed better GFP expression in Hela cell line. These results suggest that LP2ks have a potential for efficient gene delivery systems.