Development and Evaluation of bioreducible gene carrier for peptide/gene co-delivery
발표자
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초록
내용
(KLA)4 peptide has been well known as pro-apoptotic peptide, which could lead to anticancer effect through mitochondria penetration into cancer cell. However, its one-self utilization was limited due to inefficient delivery in cancer cell. To solve these problem, bioreducible gene carrier (BGC), composed with (KLA)4 peptide, water-soluble chitosan (WC) and cystamine (Cys), was synthesized by coupling agent. Its chemical structure was analyzed by 1H-NMR. BGC/gene complex was prepared by electrostatic charge interaction. Its binding affinity, DNase protection and releasing assay were carried out by gel retardation assay. Moreover, its transfection efficacy was evaluated in diverse cancer cell lines. Besides, its cytotoxicity and anticancer effect were conducted by MTT assay. These results suggest that it is superb gene carrier with targeted mitochondria and nucleus into cancer cells. (No. NRF-2016H1D5A1910499).