Design of Three Color Polymer Dots via Fluorescence Resonance Energy Transfer for Multi-Color Imaging
발표자
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초록
내용
Polymer dots (Pdots) represent fluorescent probes for bioimaging because of high brightness, low cytotoxicity, good photostability. To produce different emission wavelength of Pdots (green, red and near-infrared), we utilized green-emissive conjugated polymer alone or blend with red or NIR fluorophores to adopt fluorescence resonance energy transfer (FRET). During the formation of Pdots, polymer matrix (polystyrene maleic anhydride) was co-precipitated with fluorescent semiconducting materials, enabling efficient FRET due to 1) close distance between FRET donor and acceptor as well as 2) suppressed aggregation-caused quenching, prohibiting the serious packing of fluorophores. Subsequently, the amide bond formation between the carboxyl groups on the Pdot surface and the amine groups of target-specific antibodies was catalyzed by COMU. Upon excitation with only one wavelength, three color polymer dots can distinguish different organisms in the cell simultaneously.