Ultrasensitive organic-inorganic sandwich detection biosensor for biomedical diagnosis
발표자
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초록
내용
We present the ultrasensitive biosensors using magnetic nanoparticles combined with signal amplification via fluorescence dye encapsulated polymersomes. The magnetic nanoparticles (MNPs) are capable of seperation and purification of the targeted biomarker and polymersomes (Psomes) are easy to encapsulate molecules to generate signal. In the presence of target biomarker, that bind to the antibody-tagged MNPs and Psomes containing dye, to form a sandwich complex. We then determined the presence of biomarker after lysis the bound Psomes to release the encapsulated dye by providing one-to-multi signal. We studied organic-inorganic based platforms to diagnosis diseases with ultrasensitivity. In addition, biotin-avidin experiments were conducted to confirm whether selective separation of proteins was possible. Furthermore, it is possible to expand to various disease according to target biomarker.