Quantum dot-DNA hybrid nanogel for specific DNA assay
발표자
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초록
내용
This talk will introduce a new method to efficiently increase the DNA detection sensitivity to sub-picomolar concentrations through a short single-stranded DNA (ssDNA)-catalyzed formation of DNA hydrogel incorporating Förster resonance energy transfer (FRET) donor-acceptor pairs. A low-copy-number of ssDNA catalyzes the opening cycles of DNA hairpin loops tethered with a FRET acceptor into Y-shaped DNA structures, which are self-assembled with ssDNA-functionalized quantum dots (QDs) into a hydrogel. The self-assembled hydrogel structure of FRET pairs greatly improves FRET signals due to the close spatial proximity.