Mycobacterium Tuberculosis Detection based on FRET Particulate System with Trehalose Analogs
발표자
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초록
내용
Tuberculosis(TB) is the major global cause of death from a single infectious agent, Mycobacterium tuberculosis(Mtb). A long-time cultivation of mycobacterium is necessary to confirm tuberculosis. Although a large amount of effort is given for developing point-of care (POC) sensors, they suffers from its low sensitivity. Herein, we report a study of detecting Mycobacterium tuberculosis by FRET particulate system with trehalose analog, which is an essential metabolite in Mtb. Ag85, a major secretion protein, catalyses trehalose during cell wall synthesis. When incubated with Mtb, the detection particles exhibit a change in FRET ratio due to release of FRET dye following the rupture of particles. We optimized the particulate system for sensitivity and stability. In a pre-test, the detection system shows an increase in FRET ratio with a small amount of Ag85 protein. In subsequent experiments, the optimized particles will be incubated with mycobacterium directly.