Guiding Bone Tissue Regeneration Using Silk Fibroin-PGA Hybrid Scaffolds Prepared by Electrospinning and 3D Printing
발표자
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초록
내용
Tissue engineering aims regeneration of defected biological tissues and organs by combining cells, scaffolds, and growth factors. We developed patient-customizable guided bone regeneration (GBR)/guided tissue regeneration (GTR) hybrid scaffolds prepared by electrospinning and 3D printing techniques. Poly(glycolic acid) (PGA) scaffolds were prepared by a 3D printer. The topography of silk fibroin nanofibers and PGA scaffolds was observed by SEM. Cell seeding efficiency and proliferation of osteoblasts on a PGA scaffold were analyzed by MTT assay. Rabbit calvarial defect model was used for animal test. Bone tissue regeneration were analyzed by Masson Trichrome staining and micro-CT. Silk fibroin nanofiber-PGA hybrid scaffold groups showed significant regeneration of bone compared to control groups. The silk fibroin nanofiber-PGA scaffold construct will be an efficient approach for regeneration of bone defects.