Highly sensitive metal-enhanced fluorescence biosensor prepared on electrospun fibers decorated with silica-coated silver nanoparticles
발표자
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초록
내용
A new MEF-based biosensor platform was prepared on fibrous substrates. First, PCL fibers were obtained via electrospinning process. PCL fibers were decorated with photoreduced silver nanoparticles followed by silica coating, generating silver-decorated PCL fibers with a silica layer. The MEF was obtained from the silver-decorated PCL fibers, the extent of which could be controlled by the thickness of the silica layer. The fibrous structure of Ag@SiO2-PCL had higher protein loading capacity than conventional two-dimensional glass slides due to its large surface area, while the presence of silica-coated silver nanoparticles resulted in higher fluorescence intensity than silica-coated PCL fibers without silver nanoparticles via the MEF effect. According to immunobinding assays between fluorescently labeled anti-IgG and IgG immobilized onto different substrates, Ag@SiO2-PCL had much better sensing performance in terms of sensitivity and detection limit than glass slides and SiO2-PCL.