Nitric Oxide Releasing and Heparinized Surface for Prevention of Thrombosis and Restenosis
발표자
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초록
내용
Thrombosis and restenosis are the main obstacles in the treatment of cardiovascular diseases (CVD). Recently, nitric oxide (NO), which stimulates the endothelialization while discouraging platelet adhesion and smooth muscle cells (SMCs) activities, has emerged as a promising candidate for CDV treatment. In this study, polyvinyl chloride (PVC) substrate was immobilized with heparin and copper nanoparticles (CuNPs), a NO-catalytic agent, to reduce thrombosis and restenosis. Heparin-tyramine (HT) and CuNPs were immobilized onto PVC substrate via “one-pot” tyrosinase-mediated immobilization. The amount of immobilized heparin and released NO were controllable by varying the feeding concentration of HT and CuNPs. In vitro studies revealed that the HT/Cu coatings sustainedly released NO (0.2-18 µM for 14 days) leading to a decrease of platelets and SMCs adhesion and the enhanced endothelialization. These results demonstrated the potential use of HT/Cu coating in the cardiovascular therapy.