Preparation and evaluation of hexanoyl glycol chitosan (HGC) hydrogel coated dishes for 3D cell culture
발표자
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초록
내용
Over the past decades, three-dimensional (3D) cell culture systems have witnessed significant progress in different biomedical applications. Our previous study has given a facile method for the efficient formation of 3D spheroids using non-adhesive N-acyl glycol chitosan coated dishes. Among them, HGC is highly effective and can provide convenient and reproducible method for cell spheroids formation. However, HGC has limitations such as low gel stability and weak mechanical properties for long-term spheroid culture. In this study, chemically crosslinked HGC hydrogels were introduced to improve long-term stability of coating layer. We synthesized UV-crosslinkable methacrylated HGCs (M-HGCs) and their coating morphology was investigated. Also, cell spheroids formation cultured on coated-dishes were observed with cytotoxicity study using live/dead assay. The M-HGC coated dishes showed not only comparable spheroid forming ability but also better stability to the HGC coated dishes.