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  발표분야
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  발표구분
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Projector
  제목
Highly Aligned Polylactic-co-Glycolic Acid Microfibers for Fabricating a Multilayered Organ Chip
  공동저자
  • Minju Kim (University of Utah, 비회원)
  • Noori Na (University of Utah, 비회원)
  • Jiyoung Chang (University of Utah, 비회원)
  • Jungkyu Kim (University of Utah, 박사, 비회원)
  발표자
Jungkyu Kim (University of Utah , Email: jk**@me***********, 전화: -
  연구책임자
Jungkyu Kim (University of Utah , Email: jk**@me***********, 전화: -
  키워드
-
  우수논문발표상
우수논문발표상에 응모하지 않습니다
  발표자료 준비 관련 안내
발표자료 준비 관련 안내문을 모두 읽고 확인하였습니다.
  발표자료 수집·활용에 대한 동의서
본인은 학회 기간 동안 발표자료 수집 및 (동)영상 저장과 송출에 대하여 동의합니다.
Highly Aligned Polylactic-co-Glycolic Acid Microfibers for Fabricating a Multilayered Organ Chip
Minju Kim, Noori Na, Jiyoung Chang, Jungkyu Kim
University of Utah
To investigate cell-cell interaction and barrier functions, organ chips integrated with various porous membranes have been used for co-culture. However, these porous membranes have limited mechanical characteristics, which are low porosity, poor light transmittance, and low permeability without any cell polarity control. In this study, we present how to fabricate a highly ordered polylactic-co-glycolic acid (PLGA) microfibers membrane with high permeability to control cell polarity. Using Nearfield Electrospinning technique, about 3.14 ± 0.31 µm PLGA fibers (10 % w/w Hexafluoro-2-propanol) were formed and printed on a PEO coated Si wafer to create rectilinear grid. The following layers were created after rotating in +/- 45° to fabricate a multilayered membrane with ~13 µm pore size. This membrane then embedded into two chamber PDMS microfluidic chip. We confirmed that myofibroblasts were cultured in the chip to observe how the cells can proliferate and migrate through the fibers. With high controllability on fiber size, pore size, and permeability, this PLGA membrane embedded organ chip enables us to mimic various barrier functions to study cell-cell communication and interaction.
Keywords: -