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  발표분야
콜로이드 및 분자조립 부문위원회
  발표구분
포스터발표Poster presentation
  발표장치
Poster
  제목
Photostable polymeric nanoparticle containing hydrophobic Lumogen® dye as cellular biomarkers
  공동저자
  • 강민경 (부경대학교, 석사, 회원)
  • 김한성 (부경대학교, 박사, 비회원)
  • 이태형 (울산대학교 의과대학 의생명연구소, 비회원)
  • 허양훈 (한국기초과학지원연구원, 비회원)
  • 김영상 (부경대학교, 비회원)
  • 박선주 (부경대학교, 비회원)
  • 진준오 (영남대학교 의생명공학과, 회원)
  • 이창환 (울산대학교 의과대학 의생명연구소, 비회원)
  • 곽민석 (부경대학교, 박사, 회원)
  발표자
강민경 (부경대학교 , Email: mgkang@pukyong.ac.kr, 전화: 01022314847
  연구책임자
곽민석 (부경대학교 , Email: mkwak@pukyong.ac.kr, 전화: 0516295595
  키워드
Polymeric micelle, Lumogen, sIPN, Probe nanoparticle, Cellular uptake
  우수논문발표상
우수논문발표상에 응모하지 않습니다
Photostable polymeric nanoparticle containing hydrophobic Lumogen® dye as cellular biomarkers
강민경, 김한성, 이태형1, 허양훈2, 김영상, 박선주, 진준오3, 이창환1, 곽민석
부경대학교; 1울산대학교 의과대학 의생명연구소; 2한국기초과학지원연구원; 3영남대학교 의생명공학과
Lumogen® fluorescent dyes have great light stability as well as high fluorescence quantum yields. Despite the excellent photophysical properties of dyes, the highly hydrophobic structure in common makes the compounds difficult to function as biological fluorescence probes. To generally utilize a family of organic fluorescent dyes such as Lumogen® Red, Orange, Yellow, and Violet, as a biomarker for cellular imaging in flow cytometry and fluorescence microscopy, we used an encapsulation method using amphiphilic block copolymer namely Pluronic® F127. The photostability of Lumogen nanoparticles were compared with commercial dye, Alexa488 and DAPI, used primarily bioimaging. Cell uptake of the probe nanoparticles by HeLa cells was analyzed by flow cytometry and confocal laser scanning microscopy. Also, we observed to confirm the position of the Lumogen nanoparticles in the HeLa cells using a high voltage electron microscope.
Keywords: Polymeric micelle, Lumogen, sIPN, Probe nanoparticle, Cellular uptake